Characterization of Lactic Acid-Producing Bacteria Isolated from Rumen: Growth, Acid and Bile Salt Tolerance, and Antimicrobial Function

Lactic acid bacteria are some of the dominant in rumen, and they have a high ability for lactic production. The present study aimed to screen evaluate performance culturable rumen from Chinese Holstein dairy cows as potential probiotic or inoculant silage production, order isolate ruminal their probiotics. Three strains Enterococcus avium (E. avium, EA1-3); three Streptococcus lutetiensis (S. lutetiensis, SL1-3); six equinus equinus, SE1-6) were successfully identified fluid using modified De Man Rogosa sharp medium supplemented with 0.325% acid. E. S. clustered phylogenetic tree. All 12 Gram-positive reached plateau growth phase 6–10 h, an OD600 at about 1.8. Both gas accumulation plateaus 10–12 h all strains, showed strongest capacity. highest was detected broth (up 219.77 μmol/L). isolates inhibited pH 4.0, EA2, SL1, SL2, SL3 SE2 tolerant 0.1%, 0.2% 0.3% bile salt. In addition, supernatants had inhibitory effects on Escherichia coli Staphylococcus aureus. Specifically, exhibited inhibition pathogens. conclusion, these good inoculants probiotics edible animals, especially equinus.